质粒名称: | pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus |
---|---|
出品公司: | Addgene |
目录编号: | 61629 |
存储实验室: | Dolf Weijers |
载体骨架基本信息 |
|
载体名称: | pGreenII |
载体出品公司: | -- |
载体抗性: | Kanamycin low (25 ug/ml) |
空载体大小: | 2580 bp |
完整质粒大小: | 12791 bp |
载体修饰: | -- |
载体类型: | 植物表达 |
筛选标记: | Methothrexate |
高拷贝/低拷贝: | 低拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
|
插入基因名称: | mDII,Fragment of Arabidopsis IAA28, mutated to prevent auxin-dependent degradation |
物种来源: | A. thaliana (mustard weed) |
插入大小: | 102 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 |
RPS5A
|
标签/融合蛋白: | -- |
克隆方法: | Ligation Independent克隆 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | M13_forward20_primer |
3' 测序引物 | -- |
文献引用方法 |
|
材料和方法部分: | pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus was a gift from Dolf Weijers (Addgene plasmid # 61629 ; http://n2t.net/addgene:61629 ; RRID:Addgene_61629) |
参考文献部分: | Reporters for sensitive and quantitative measurement of auxin response. Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D. Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2. 10.1038/nmeth.3279 PubMed 25643149 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus 12920 bp ds-DNA circular SYN 27-FEB-2019 DEFINITION R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter. ACCESSION . VERSION . KEYWORDS pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12920) AUTHORS Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D TITLE Reporters for sensitive and quantitative measurement of auxin response. JOURNAL Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2. PUBMED 25643149 REFERENCE 2 (bases 1 to 12920) AUTHORS . TITLE Direct Submission JOURNAL Exported Feb 27, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12920 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(1721..1739) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 1752..1776 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1912..1932 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1933..3363 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" promoter complement(5352..5370) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 5383..5407 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5543..5563 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5594..6310 /codon_start=1 /product="Venus YFP with monomerizing A206K mutation (Nagai et al., 2002)" /label=mVenus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6329..6349 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6380..7096 /codon_start=1 /product="Venus YFP with monomerizing A206K mutation (Nagai et al., 2002)" /label=mVenus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 7130..7849 /codon_start=1 /product="Venus YFP with monomerizing A206K mutation (Nagai et al., 2002)" /label=mVenus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(8133..8153) /label=T3 /note="T3 promoter, forward primer" promoter complement(8133..8151) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8172..8188) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8172..8188) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8185..8207) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8196..8212 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8220..8250) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8265..8286 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8403..8420) /label=L4440 /note="L4440 vector, forward primer" misc_feature 8525..8549 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" rep_origin complement(8640..9228) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8720..8739) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9399..10214) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 10122..10141 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 10206..10225 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin 10505..10940 /label=pSa ori /note="origin of replication from bacterial plasmid pSa" misc_feature 11067..11089 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA (truncated)" polyA_signal 11097..11273 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(11669..12232) /codon_start=1 /gene="Mus musculus Dhfr" /product="mouse dihydrofolate reductase" /label=DHFR /translation="MVRPLNCIVAVSQNMGIGKNGDRPWPPLRNEFKYFQRMTTTSSVE GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY PGVLSEVQEEKGIKYKFEVYEKKD" promoter complement(12265..12610) /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind complement(12302..12322) /label=35S promoter /note="CaMV 35S promoter, forward primer" primer_bind 12813..12835 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 12827..12844 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 12828..12844 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 12854..12872 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 tagttggaat aggatttccc gggccataat cgtgagtaga tatattactc aacttttgat 61 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtggcactg tttgcactgg 121 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac 181 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt 241 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat 301 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga 361 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgcttca gccatatcac 421 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt 481 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt 541 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc 601 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct 661 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt 721 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa 781 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag 841 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca 901 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg 961 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta 1021 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt 1081 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa 1141 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta 1201 tgatcactga cttggttcta accaaaaaaa aaaatgttta atttacatat ctaaaagtag 1261 gtttagggaa acctaaacag taaaatattt gtatattatt cgaatttcac tcatcataaa 1321 aacttaaatt gcaccataaa attttgtttt actattaatg atgtaatttg tgtaacttaa 1381 gataaaaata atattccgta agttaaccgg ctaaaaccac gtataaacca gggaacctgt 1441 taaaccggtt ctttactgga taaagaaatg aaagcccatg tagacagctc cattagagcc 1501 caaaccctaa atttctcatc tatataaaag gagtgacatt agggtttttg ttcgtcctct 1561 taaagcttct cgttttctct gccgtctctc tcattcgcgc gacgcaaacg atcttcaggt 1621 gatcttcttt ctccaaatcc tctctcataa ctctgatttc gtacttgtgt atttgagctc 1681 acgctctgtt tctctcacca cagccgggta cccaattcgc cctatagtga gtcgtattac 1741 aattcactgg cccaagtttg tacaaaaaag caggctatga aacaaaaaag ctcgaccaaa 1801 gaaacatcat tcctttccaa taacagggtt gaggtagctc cagtggtggg atggctgccg 1861 gtgagatcat cccggagagg gaaatccaac tcaatactga agcttatggc tccaaagaag 1921 aagagaaagg tcatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag 1981 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc 2041 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc 2101 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac 2161 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc 2221 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac 2281 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta 2341 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc 2401 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg 2461 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg 2521 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag 2581 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc 2641 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc 2701 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc 2761 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc 2821 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg 2881 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg 2941 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg 3001 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc 3061 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc 3121 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac 3181 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac 3241 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag 3301 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag 3361 tagctcgaga gatccgatcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt 3421 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca 3481 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt 3541 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa 3601 attatcgcgc gcggtgtcat ctatgtgaat tctagttgga atagggttcg ggccataatc 3661 gtgagtagat atattactca acttttgatt cgctatttgc agtgcacctg tggcgttcat 3721 cacatctttt gtggcactgt ttgcactggt cattgctatt acaaaggacc ttcctgatgt 3781 tgaaggagat cgaaagtaag taactgcacg cataaccatt ttctttccgc tctttggctc 3841 aatccatttg acagtcaaag acaatgttta accagctccg tttgatatat tgtctttatg 3901 tgtttgttca agcatgttta gttaatcatg cctttgattg atcttgaata ggttccaaat 3961 atcaaccctg gcaacaaaac ttggagtgag aaacattgca ttcctcggtt ctggacttct 4021 gctagtaaat tatgcttcag ccatatcact agctttctac atgcctcagg tgaattcatc 4081 tatttccgtc ttaactattt cggttaatta aagcacgaac accattactg catgtagaag 4141 cttgataaac tatcgccacc aatttatttt tgttgcgata ttgttacttt cctcagtatg 4201 cagctttgaa aagaccaacc ctcttatcct ttaacaatga acaggttttt agaggtagct 4261 tgatgattcc tgcacatgtg atcttggctt caggcttaat tttccaggta aagcattatg 4321 agatactctt atatctctta catacttttg agataatgca caagaacttc ataactatat 4381 gctttagttt ctgcatttga cactgccaaa ttcattaatc tctaatatct ttgttgttga 4441 tctttggtag acatgggtac tagaaaaagc aaactacacc aaggtaaaat acttttgtac 4501 aaacataaac tcgttatcac ggaacatcaa tggagtgtat atctaacgga gtgtagaaac 4561 atttgattat tgcaggaagc tatctcagga tattatcggt ttatatggaa tctcttctac 4621 gcagagtatc tgttattccc cttcctctag ctttcaattt catggtgagg atatgcagtt 4681 ttctttgtat atcattcttc ttcttctttg tagcttggag tcaaaatcgg ttccttcatg 4741 tacatacatc aaggatatgt ccttctgaat ttttatatct tgcaataaaa atgcttgtac 4801 caattgaaac accagctttt tgagttctat gatcactgac ttggttctaa ccaaaaaaaa 4861 aaaaatgttt aatttacata tctaaaagta ggtttaggga aacctaaaca gtaaaatatt 4921 tgtatattat tcgaatttca ctcatcataa aaacttaaat tgcaccataa aattttgttt 4981 tactattaat gatgtaattt gtgtaactta agataaaaat aatattccgt aagttaaccg 5041 gctaaaacca cgtataaacc agggaacctg ttaaaccggt tctttactgg ataaagaaat 5101 gaaagcccat gtagacagct ccattagagc ccaaacccta aatttctcat ctatataaaa 5161 ggagtgacat tagggttttt gttcgtcctc ttaaagcttc tcgttttctc tgccgtctct 5221 ctcattcgcg cgacgcaaac gatcttcagg tgatcttctt tctccaaatc ctctctcata 5281 actctgattt cgtacttgtg tatttgagct cacgctctgt ttctctcacc acagccgggt 5341 acccaattcg ccctatagtg agtcgtatta caattcactg gcccaagttt gtacaaaaaa 5401 gcaggctatg aaacaaaaaa gctcgaccaa agaaacatca ttcctttcca ataacagggt 5461 tgaggtagct ccagtggtgg gatggccgcc ggtgagatca tcccggagag aacccaactc 5521 catactggat ccaacaatgg ctcccaagaa gaagagaaag gtagatcccc gggctgcagg 5581 aattaattcg atcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 5641 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 5701 cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt 5761 gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc 5821 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 5881 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 5941 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 6001 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga 6061 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg 6121 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 6181 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg 6241 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 6301 gctgtacaag ttagatccaa caatggctcc caagaagaag agaaaggtag atccccgggc 6361 tgcaggaatt aattcgatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 6421 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 6481 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct 6541 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg 6601 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 6661 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 6721 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 6781 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac 6841 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga 6901 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 6961 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 7021 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 7081 ggacgagctg tacaagttag atccaggagc aggagcagga gcaggagcca tggtgagcaa 7141 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 7201 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 7261 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 7321 cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt 7381 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 7441 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 7501 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 7561 caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc 7621 caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgctg accactacca 7681 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta 7741 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 7801 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag atcctctaga 7861 gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat 7921 aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt 7981 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa 8041 tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcaagctta tctagagcgg 8101 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg 8161 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 8221 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 8281 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 8341 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 8401 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 8461 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgaag 8521 gccttgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt ttgagatctc 8581 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 8641 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 8701 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 8761 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 8821 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 8881 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 8941 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 9001 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 9061 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 9121 ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 9181 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 9241 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 9301 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 9361 tcaatctaaa gtatatatgt gtaacattgg tctagtgatt agaaaaactc atcgagcatc 9421 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt 9481 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat 9541 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa 9601 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa 9661 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa 9721 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg 9781 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact 9841 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct 9901 gttttccctg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc 9961 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta 10021 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc 10081 ccatacaatc ggtagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac 10141 ccatataaat cagcatccat gttggaattt aatcgcggcc ttgagcaaga cgtttcccgt 10201 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt 10261 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg 10321 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac 10381 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac 10441 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcg 10501 atccccatcc aacagcccgc cgtcgagcgg gcttttttat ccccggaagc ctgtggatag 10561 agggtagtta tccacgtgaa accgctaatg ccccgcaaag ccttgattca cggggctttc 10621 cggcccgctc caaaaactat ccacgtgaaa tcgctaatca gggtacgtga aatcgctaat 10681 cggagtacgt gaaatcgcta ataaggtcac gtgaaatcgc taatcaaaaa ggcacgtgag 10741 aacgctaata gccctttcag atcaacagct tgcaaacacc cctcgctccg gcaagtagtt 10801 acagcaagta gtatgttcaa ttagcttttc aattatgaat atatatatca attattggtc 10861 gcccttggct tgtggacaat gcgctacgcg caccggctcc gcccgtggac aaccgcaagc 10921 ggttgcccac cgtcgagcgc ctttgcccac aacccggcgg ccggccgcaa cagatcgttt 10981 tataaatttt tttttttgaa aaagaaaaag cccgaaaggc ggcaacctct cgggcttctg 11041 gatttccgat ccccggaatt agatcttggc aggatatatt gtggtgtaac gttatcgatc 11101 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt 11161 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata 11221 agaaccctaa tttcccttat cgggaaacta ctcacacatt atttatggag aaaatagaga 11281 gagatagatt tgtagagaga gactggtgat ttcagcgtac cgaattaatt cgagctcggt 11341 acccctggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac 11401 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taagaaccct 11461 aattccctta tctgggaact actcacacat tattatagag agagatagat ttgtagagag 11521 agactggtga tttcagcggg ggattcagcg ccgacgggac gtaaacaaag gacgtcccgc 11581 gaaggatcta aagccagcaa aagtcccatg gtcttataaa aatgcatagc tttaggaggg 11641 gagcagagaa cttgaaagca tcttcctgtt agtctttctt ctcgtagact tcaaacttat 11701 acttgatgcc tttttcctcc tggacctcag agaggacgcc tgggtattct gggagaagtt 11761 tatatttccc caaatcaatt tctgggaaaa acgtgtcact ttcaaattcc tgcatgatcc 11821 ttgtcacaaa gagtctgagg tggcctggtt gattcatggc ttcctggtaa acagaactgc 11881 ctccgactat ccaaaccatg tctactttac ttgccaattc cggttgttca ataagtctta 11941 aggcatcatc caaacttttg gcaagaaaat gagctcctcg tggtggttct ttgagttctc 12001 tactgagaac tatattaatt ctgtccttta aaggtcgatt cttctcagga atggagaacc 12061 aggttttcct acccataatc accagattct gtttaccttc cactgaagag gttgtggtca 12121 ttctttggaa gtacttgaac tcgttcctga gcggaggcca gggtcggtct ccgttcttgc 12181 caatccccat attttgggac acggcgacga tgcagttcaa tggtcgaacc atgatggcaa 12241 attctagggg tacccagctt gggctgtcct ctccaaatga aatgaacttc cttatataga 12301 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatgtc 12361 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct 12421 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa tgatagcctt 12481 tcctttatcg caatgatggc atttgtagga gccaccttcc ttttctactg tcctttcgat 12541 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgaaatta ccctttgttg 12601 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgacatttt tggagtaggg 12661 gtacgataac attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga 12721 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 12781 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 12841 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc ccgtatggtt 12901 aattaagaca caggcgcgcc //